在S.S.中识别了1600个复制起源. 类植物
Eric J Foss1, Carmina Lichauco1, Tonibelle Gatbonton-Schwager1
1Clinical Research Division, Fred Hutch Cancer Center, Seattle, United States.
eLife
|February 5, 2024
概括
酵母DNA复制的起源比以前认为的要丰富得多,Mcm结合点表明了整个基因组的潜在启动. 这表明复制可能发生在大多数基因间区域,模糊与人类DNA复制的区别.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 细胞生物学 细胞生物学
背景情况:
- 酵母基因组有大约500个已知的DNA复制起源,其中启动是由S阶段之前加载的Mcm复制酶促进的.
- 一项先前的研究将复制起源定义为G1中绑定Mcm的位点,通常是单个Mcm双六合体,与ARS共识序列 (ACS) 相邻.
研究的目的:
- 将Mcm结合点的分析扩展到整个酵母基因组,以确定候选复制来源.
- 调查Mcm丰富度,复制启动和其他基因组中的起源标志之间的关系.
主要方法:
- 用微型菌核酶与Mcm子单元结合而形成的Mcm结合位点的全基因组识别.
- 对单链DNA (ssDNA) 数据的分析,以将Mcm丰富度与复制启动相关联.
- 核细胞定位的评估,ACS的存在,并在Mcm结合部位的GC倾斜.
主要成果:
- 确定了候选MCM结合点,信号强度的变化超过3个数量级.
- 通过ssDNA表示的复制启动,在最丰富的Mcm结合位点 (前1600位) 检测到,随着Mcm丰富度的下降.
- 最多的5500 Mcm结合位点表现出复制起源的特征,包括基因间位置,侧边核体,ACS存在和GC倾斜.
结论:
- 酵母中的DNA复制起源至少比以前假设的多出三倍,Mcm加载地点可能作为起源.
- 复制可能在几乎每一个基因间区域启动,与最近发现的已知来源之外的启动结果一致.
- 酵母复制起源的更广泛分布表明,它们与人类S阶段的相似性比以前认为的更大.
关键词:
复制DNA复制DNA复制DNA复制麦克姆麦克姆是什么意思它们中的一种是S. cerevisiae.染色体是一种染色体.基因表达的基因表达方式遗传学 遗传学 遗传学 是一个基因组学就是基因组学.螺旋酸的使用方法复制启动复制的开始.复制的起源 复制的起源更多相关视频
08:40Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
Published on: October 21, 2022
1.5K
08:53Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
Published on: May 2, 2025
357
相关概念视频
Chromosome Structure
22.8K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
22.8K
Chromosome Replication
8.7K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins. This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
8.7K
Replication in Eukaryotes
13.8K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
13.8K
S-Cdk Initiates DNA Replication
4.7K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
4.7K
The DNA Replication Fork
36.0K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
36.0K
Replication in Prokaryotes
24.9K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
24.9K
