哺乳动物抗原显示用于流行病对策
Andrea Quezada1, Ankur Annapareddy1, Kamyab Javanmardi1
1Department of Molecular BioSciences, University of Texas at Austin, Austin, TX, USA.
Methods in molecular biology (Clifton, N.J.)
|February 5, 2024
概括
这项研究介绍了一种哺乳动物细胞表面显示平台,用于快速抗原设计和分析. 这种方法加速了疫苗开发和病毒监测,克服了以前基于酵母的系统的局限性.
科学领域:
- 生物技术是生物技术.
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 快速抗原设计对于抗疫措施至关重要,包括疫苗开发和病毒监测.
- 目前的细胞表面显示方法,通常使用酵母,与大型病毒葡萄糖蛋白作斗争.
- 有效地将蛋白质变体与它们的DNA序列联系起来,对于高通量选至关重要.
研究的目的:
- 为病毒抗原开发一个哺乳动物细胞表面显示平台.
- 为了克服酵母显示表达大型病毒蛋白的局限性.
- 为了促进快速抗原设计,抗体反应概况和病毒系监测.
主要方法:
- 在哺乳动物HEK293T细胞表面设计和表达病毒抗原.
- 利用流式细胞计量来选表面显示的蛋白质,绕过净化步骤.
- 应用了选稳定突变,深度突变扫描和表位图绘制的平台.
主要成果:
- 在哺乳动物细胞表面上成功证明了抗原表达.
- 在三个不同的用例中展示了平台的实用性.
- 在没有蛋白质净化的情况下验证了选的效率.
结论:
- 哺乳动物抗原显示平台加速了病毒抗原的设计和分析.
- 这项技术增强了疫情防控和应对能力.
- 能够更快地开发疫苗和诊断工具.
相关概念视频
Antigens Involved in Adaptive Immunity
496
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
Complete Antigens
Complete antigens possess both immunogenicity and...
496
Antigen Processing Pathways
1.0K
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
MHC Class I: Presenting Endogenous...
1.0K
Vaccinations
44.5K
Overview
44.5K
Antigen Presenting Cells
1.8K
The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
1.8K


