Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
Proteomics01:33

Proteomics

7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Ligand selectivity and competitive adsorption of heavy metals using beta cyclodextrin-based metal organic framework and crosslinked polymers.

Scientific reports·2026
Same author

Cnidarian-algal partnerships structure bacterial communities during strobilation in <i>Cassiopea xamachana</i>.

ISME communications·2026
Same author

Deep eutectic solvent-assisted extraction enhances functionality of yellow split pea protein: A sustainable approach for food applications.

International journal of biological macromolecules·2026
Same author

Metabolic Remodeling of the Parkinson's Disease Frontal Cortex Revealed by LC-MS/MS Metabolomics.

Biomolecules·2026
Same author

High-resolution LC-MS/MS analysis of brain N-glycans reveals composition-specific changes in Parkinson's disease.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same author

Qualitative Characterization of Neu5Gc-Containing N-Glycans in Commercial Cheddar Cheese Products Using Nano-UHPLC-ESI-MS/MS-A Pilot Study.

International journal of molecular sciences·2026

相关实验视频

Updated: Jul 4, 2025

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

2.4K

使用MRM/PRM方法进行向性糖蛋白组学分析.

Cristian D Gutierrez Reyes1, Akeem Sanni1, Moyinoluwa Adeniyi1

  • 1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.

Methods in molecular biology (Clifton, N.J.)
|February 5, 2024
PubMed
概括

质谱 (MS) 定向分析,包括多重反应监测 (MRM) 和并行反应监测 (PRM),验证生物分子结构变化. 这些有针对性的方法通过提高选择性和特异性来增强复杂的生物样本中的糖蛋白组学分析.

关键词:
葡萄糖是一种糖.这是一个MRMM.在PRM中,PRM是PRM.

更多相关视频

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

10.1K
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

1.0K

相关实验视频

Last Updated: Jul 4, 2025

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

2.4K
An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

10.1K
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

1.0K

科学领域:

  • 生物化学和分析化学的研究.
  • 生物分子分析.
  • 蛋白质组学和糖组学.

背景情况:

  • 用质谱法 (MS) 准的分析对于研究生物分子的结构变化至关重要.
  • 蛋白质组学,糖组学,脂组学和代谢组学等领域广泛使用这些技术.
  • 多重反应监测 (MRM) 和并行反应监测 (PRM) 是常见的有针对性的研究方法.

研究的目的:

  • 突出MS针对性分析在生物分子研究中的实用性.
  • 强调MRM和PRM技术在有针对性的研究中的作用.
  • 展示这些技术在糖蛋白组学分析中的好处.

主要方法:

  • 使用质谱学 (MS) 针对性分析.
  • 使用多重反应监测 (MRM) 和并行反应监测 (PRM) 技术.
  • 应用这些方法来分析生物分子的结构变化.

主要成果:

  • 针对MS的分析有效验证生物分子的结构变化.
  • MRM和PRM技术简化了分析工作流程.
  • 这些方法在复杂的生物矩阵中增强了选择性和特异性.

结论:

  • 针对MS的分析,特别是MRM和PRM,是生物分子研究的重要工具.
  • 这些技术提供了可靠和高效的糖蛋白组学分析.
  • 这些方法提高了准确性,并减少复杂样品的背景干扰.