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使用纳米HPLC和Tandem MS进行O-Glycoproteomics样本的准备和分析

Junyao Wang1, Sherifdeen Onigbinde1, Waziha Purba1

  • 1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.

Methods in molecular biology (Clifton, N.J.)
|February 5, 2024
PubMed
概括

这项研究详细介绍了O链 glycoproteomics 的自下而上的方法,这是一个关键的翻译后修改影响蛋白质功能和疾病. 该方法使用酶消化和质谱仪进行精确的O-糖分析.

关键词:
葡萄糖基酶化是什么? 葡萄糖基酶化在O-Glycoprotease的使用中.与O链接的葡萄糖.在 RPLC 中,使用 RPLC 系统.两者并排的MSMS是什么

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