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相关概念视频

Protein Glycosylation01:25

Protein Glycosylation

6.9K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
6.9K
Conjugated Proteins02:50

Conjugated Proteins

18.3K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
18.3K

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相关实验视频

Updated: Jul 4, 2025

Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells
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Rapid Antibody Glycoengineering in Chinese Hamster Ovary Cells

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对糖蛋白生产的考虑因素

Elizabeth C Clarke1

  • 1Center for Global Health, Division of Infectious Diseases, Department of Internal Medicine, University of New Mexico, Albuquerque, NM, USA. eclarke@salud.unm.edu.

Methods in molecular biology (Clifton, N.J.)
|February 5, 2024
PubMed
概括

选择用于重组糖蛋白生产的正确表达系统是关键. 糖化在诸如大肠杆菌,昆虫,酵母和哺乳动物细胞等系统之间有很大的差异,影响蛋白质功能.

科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 糖蛋白的生产是复杂的,因为由酶驱动的糖化,而不是直接的遗传控制.
  • 宿主细胞系统的进化差异导致了不同的糖化模式.
  • 了解甘氨基化对于选择适当的蛋白质生产系统至关重要.

研究的目的:

  • 引导研究人员选择复合甘氨酸蛋白的最佳表达系统.
  • 突出不同表达系统对糖系化模式的影响.
  • 根据预期的应用和所需的蛋白质特征进行明智的选择.

主要方法:

  • 在各种表达系统中对糖化酶的比较分析.
  • 审查已建立的系统,包括大肠杆菌,细菌病毒/昆虫细胞,Pichia pastoris和哺乳动物细胞 (CHO,HEK,BHK).
  • 考虑生长条件及其对糖化酶的影响.

主要成果:

  • 在 prokaryotic,酵母和哺乳动物表达平台之间存在 glycosylation 概况的显著差异.
  • 系统的选择对重组甘氨酸蛋白的最终甘氨酸结构有深远的影响.
  • 特定的系统可能更适合模仿原生糖化或工程糖形.
关键词:
细菌病毒病毒的病毒.中国仓鼠的卵巢细胞.复杂的甘氨酸化合物埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.表达系统的表达方式甘氨酸是什么 甘氨酸是什么 甘氨酸葡萄糖蛋白质是一种糖蛋白质.葡萄糖基酶化是什么? 葡萄糖基酶化人体内皮的脏通过N链接的糖基化.与O相关的葡萄糖化.这就是Pichia pastoris.重组蛋白质生产的重组蛋白质生产

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High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
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Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
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Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

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High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
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结论:

  • 表达系统的选择对重组糖蛋白质的质量和功能产生了重大影响.
  • 研究人员在选择生产平台时必须考虑糖化机械和进化分歧.
  • 根据应用 (例如疫苗) 量身定制的系统选择允许优化糖蛋白特性.