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外源硫化通过阿拉比多普西斯拼接因子AtU2AF65a促进植物开花

Tian Ma¹, Shutian Xu¹, Yaqin Wang¹

¹School of Life Science, Shanxi Key Laboratory for Research and Development of Regional Plants, Shanxi University, Taiyuan, China.

Plant, cell & environment

|February 5, 2024

概括

硫化 (H2S) 通过调节开花基因的替代拼接,促进植物的开花. 这发生在拼接因子AtU2AF65a的持续硫化过程中,影响其RNA结合.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

替代拼接 (AS) 是一个关键的转录后调节机制,控制植物的花过渡.
硫化 (H2S) 是一种气体传递物,具有至关重要的生理作用,可能通过蛋白质化作用.
AS和H2S都与调节植物开花有关,拼接因素是必不可少的.

研究的目的:

调查H2S在调节Arabidopsis.com的开花时间中的作用.
为了确定H2S是否影响与开花相关的基因的替代拼接.
阐明H2S通过拼接因子AtU2AF65a影响开花的分子机制.

主要方法:

阿拉比多普西斯塔利亚纳的突变分析 (atu2af65a-4).
转录组测序以分析替代拼接模式.
透硫试验检测通过H2S检测蛋白质的修饰.
RNA免疫沉以评估蛋白质-RNA相互作用.

主要成果:

在atu2af65a-4突变体中,H2S促进开花的作用被废除了.
在AtU2AF65a依赖的方式中,H2S显著改变了开花基因 (例如SPA1,LUH,LUG,MAF3) 的替代拼接.
发现AtU2AF65a被H2S继续硫化,而H2S改变了它与开花基因前mRNA的结合亲和力.

结论:

在Arabidopsis.S中,H2S调节了与开花相关的基因的替代拼接.
这种调节通过合因子AtU2AF65a的持续硫化进行调节.
由H2S诱导的AtU2AF65a持续硫化导致改变了mRNA前剪接,最终促进了早期开花.

关键词:

替代性拼接是一种替代性的拼接.花的过渡期花的过渡期与开花相关的基因在 pre-mRNA 中.

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