相关实验视频

Updated: Jul 4, 2025

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

在M1巨细胞极化中,ROS-ATM-Chk2轴介导的代谢和细胞周期重编程的新作用

Chunlu Li¹, Chengsi Deng¹, Siwei Wang¹

¹The College of Basic Medical Science, Health Sciences Institute, China Medical University, Shenyang, Liaoning Province, China; Key Laboratory of Cell Biology of Ministry of Public Health, Key Laboratory of Medical Cell Biology of Ministry of Education, Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors of Ministry of Education, Liaoning Province Collaborative Innovation Center of Aging Related Disease Diagnosis and Treatment and Prevention, China Medical University, Shenyang, Liaoning Province, China.

|February 5, 2024

概括

反应性氧物种 (ROS) 通过DNA损伤反应 (DDR) 激酶ATM和Chk2.2信号M1巨细胞两极分化. 抑制这种ROS-Chk2通路可以减少肺炎,为炎症性疾病提供潜在的治疗方法.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

反应性氧物种 (ROS) 在巨细胞介导的炎症中至关重要.
将ROS与巨细胞两极分化联系在一起的确切分子机制尚未完全理解.

研究的目的:

阐明ROS在调节巨细胞极化中的作用.
确定涉及ROS介导的M1巨细胞两极化中的特定分子通路.

主要方法:

研究了涉及ROS,ATM和Chk2的信号通路在巨细胞极化中.
利用分子分析来确定PKM2的Chk2酸化及其对糖解的影响.
研究了Chk2对p21表达和细胞循环停止的影响.
在感染LPS的Chk2-缺乏小鼠中评估肺炎和M1巨细胞计数.

主要成果:

ROS 作为信号分子,通过 ATM 和 Chk2 激酶调节 M1 巨细胞极化.
Chk2酸化PKM2,增强糖解并促进M1的极化.
Chk2激活上调 p21,诱导细胞循环停止,这对M1极化至关重要.
在LPS挑战时,Chk2缺乏的小鼠显示肺炎和M1巨细胞数量减少.

结论:

ROS-Chk2轴是M1巨细胞极化的一个关键调节器.
准ROS-Chk2通路为炎症性疾病提供了潜在的治疗策略.
这项研究扩大了对天生的免疫力中DNA损伤反应 (DDR) 的理解.

关键词:

在 Chk2 中使用 Chk2.巨细胞是一个巨细胞.在PKM2中,PKM2是PKM2.这就是ROSOS ROS.在p21中,p21是什么?

更多相关视频

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Published on: April 18, 2025

相关概念视频

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Cell Polarization by Rho Proteins

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...