基于共价有机框架的高性能纳米复合材料用于ATP传感器的构建
Zhiyi Xi1, Juan Xing1, Ruo Yuan1
1Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.
Biosensors & bioelectronics
|February 5, 2024
概括
一种由共价有机框架和碳纳米管组成的新型纳米复合材料增强了超敏感生物传感器的光电活性. 该传感器使用DNA放大检测痕迹ATP,实现高灵敏度和准确性用于体外诊断.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术 纳米技术
- 分析化学 分析化学
背景情况:
- 开发敏感和选择性的生物传感器对于早期疾病诊断至关重要.
- 光电化学 (PEC) 生物传感器在灵敏度和实时检测方面具有优势.
- 共价有机框架 (COF) 和单壁碳纳米管 (SWCNT) 是提高PEC性能的有希望的材料.
研究的目的:
- 开发一种高性能光电纳米复合材料 (COF/SWCNT),以改善电荷分离和载体迁移.
- 制造一种超敏感的PEC生物传感器,用于微量检测腺三酸盐 (ATP).
- 整合一个以EXO III为辅助的双重DNA循环放大策略,以增强信号生成.
主要方法:
- 合成了一种新的TAPT-TFPBCOF,并将其装饰在SWCNT上,以创建一个COF/SWCNT纳米复合材料.
- 在PEC生物传感器设置中使用了COF/SWCNT纳米复合材料.
- 采用Exo III辅助的双重DNA循环放大,并与杂交连锁反应 (HCR) 进行信号放大.
- 嵌入的甲 (MnPP) 作为一个火器来调节光电流.
主要成果:
- 这种COF/SWCNT纳米复合材料显著改善了接口电荷分离和光生成载体迁移.
- 该PEC生物传感器显示了ATP的广泛检测范围,从10 fmol L-1到10 nmol L-1.1.
- 获得了2.75 fmol L-1 (S/N = 3) 的ATP的低检测极限.
- 生物传感器表现出极好的稳定性,特异性和可重复性.
结论:
- 开发的COF/SWCNT纳米复合材料有效提高PEC性能.
- 拟议的具有DNA放大策略的PEC生物传感器可实现超敏感且准确的痕迹ATP检测.
- 这项工作为敏感和可靠的体外诊断提供了一个有希望的平台.
相关概念视频
01:48ATP Synthase: Mechanism
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased ATP...
01:18ATP Synthase: Structure
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...