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鲁比斯科积累因子1-样 (RAFL) 与RAF1相互作用,以调解Arabidopsis中的鲁比斯科组合

Yuting Cheng¹, Bing Yang¹, Ting Jia²

¹Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou, 225009, China; College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, China.

Biochemical and biophysical research communications

|February 5, 2024

概括

鲁比斯科积累因子1-like (RAFL) 和鲁比斯科积累因子1 (RAF1) 是类似的蛋白质,它们介导了Arabidopsis中的鲁比斯科组装. RAFL和RAF1形成二元体,对鲁比斯科生物发生和植物生存至关重要.

科学领域:

植物分子生物学植物分子生物学
蛋白质的生物化学蛋白质的生物化学
光合作用研究研究光合作用.

背景情况:

鲁比斯科 (ribulose-1,5-bisphosphate carboxylase/oxygenase) 是光合作用中的一个关键酶,其适当的组合对植物生长至关重要.
鲁比斯科组件需要特定的陪伴者,包括鲁比斯科积累因子1 (RAF1),它稳定了RbcL二次元.
玉米中RAF1的丧失导致鲁比斯科缺乏和苗木死亡,突出了其至关重要的作用.

研究的目的:

为了研究RAF1同类的功能和特征,RAF1-like (RAFL),在Arabidopsis.
确定RAFL和RAF1.1之间的进化关系和相互作用.

主要方法:

在RAFL和RAF1.1之间进行序列相似性分析和保存域识别.
预测3D结构和分析基因表达模式.
遗传学树的构建,以了解进化起源.
在体内和体外的蛋白质与蛋白质相互作用测试以评估RAFL-RAF1相互作用.

主要成果:

RAFL与RAF1具有显著的序列相似性 (61.98%) 和保存域,具有类似的预测结构和表达模式.
遗传学分析表明,RAFL和RAF1存在于双叶植物中,其他植物系中只有一种RAF副本.
多种相互作用方法证实RAFL和RAF1在体内和体外都有相互作用.

结论:

RAFL和RAF1被确定为参与Arabidopsis中的Rubisco组装的近似基因.
这些蛋白质可以形成异构体和/或同构体,在Rubisco生物发生中发挥着至关重要的作用.
这些发现为Rubisco组装的分子机制及其在植物中的进化保存提供了洞察力.

关键词:

阿拉比多普西斯 (Arabidopsis) 是一种植物.相似的基因相似的基因蛋白质与蛋白质的相互作用鲁比斯科 (Rubisco) 是一个古老的葡萄酒.鲁比斯科的积累因子1 (RAF1)

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