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相关概念视频

Positive Regulator Molecules01:45

Positive Regulator Molecules

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To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Regulation of Metabolism01:19

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Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
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The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
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相关实验视频

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A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
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一个自我调节的网络,用于在复杂的生物合成途径中动态平衡多种前体.

Yusong Zou1, Jianli Zhang1, Jian Wang1

  • 1School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA, 30602, USA.

Metabolic engineering
|February 5, 2024
PubMed
概括

这项研究引入了一个自我调节的微生物网络来管理竞争的代谢途径. 这种动态平衡通过优化前体流量来增强像4-基胺 (4-HC) 这样的复杂分子的产生.

关键词:
4 - - 氧玛林是一种4-氧玛林.生物合成生物合成动态调节 动态调节遗传生物传感器 遗传生物传感器多个前体的前体.

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科学领域:

  • 合成生物学 合成生物学
  • 代谢工程是代谢工程.
  • 生物化学工程 生物化学工程

背景情况:

  • 微生物合成为传统方法提供了可持续的替代方案,但与竞争的代谢途径面临挑战.
  • 对细胞资源的竞争和碳流冲突可以降低目标产品生物合成的效率和产量.
  • 复杂的合成途径需要来自共享代谢节点的多种前体,加剧了这些生产限制.

研究的目的:

  • 开发一个自我监管网络,以减轻复杂微生物合成途径中的前体竞争.
  • 为了动态控制代谢流和基因表达,提高生产效率.
  • 为了证明这种方法的有效性,使用4-基氨酸 (4-HC) 合成途径作为模型系统.

主要方法:

  • 设计了一个使用中间代谢物作为触发器的自我调节网络.
  • 动态重新连接的pyruvate的代谢流和控制4-HC通路中的基因表达.
  • 采用转录基因分析来监测基因表达的变化,以应对中间度.

主要成果:

  • 在4-HC合成途径中成功实现了多种前体的自我调节.
  • 通过动态代谢流量控制,证明了4-HC的增强产量标位.
  • 转录组数据证实了酸盐激酶 (PykF) 和SdgA基因表达的动态变化,与中间水平相关.

结论:

  • 建立了一个自我调节的网络,能够动态平衡用于前体生物合成的竞争代谢流.
  • 开发的系统为优化微生物产生需要复杂代谢途径的化合物提供了一种新的策略.
  • 提供了关于管理前体干扰和微生物生物合成竞争的见解,以提高产量.