在市政废水中对人类口腔病毒的分子表征,使用安普利康目标测序
Liang Peng1, Fengxia Yang2, Jingliang Shi1
1College of Environmental Science and Engineering, Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Nankai University, Tianjin 300071, China.
The Science of the total environment
|February 5, 2024
概括
人口口腔病毒 (HBoV) 在废水中普遍存在,其中基因型HBoV2和HBoV3是最常见的. 使用安普利康序列的废水分析有效地检测到HBoV,有助于了解其传播和识别新的病毒株.
科学领域:
- 环境微生物学环境微生物学
- 病毒学 病毒学
- 公共卫生 公共卫生
背景情况:
- 人类口腔病毒 (HBoV) 是一种新兴的病原体,与胃肠炎和呼吸系统疾病有关.
- 了解废水等环境系统中HBoV的流行率对于公共卫生至关重要.
- 污水处理厂 (WWTP) 为传染病原体提供了有价值的监测点.
研究的目的:
- 调查HBoV在WWTP的发生,流行和持续情况.
- 评估安普利康目标测序,以便在废水中直接检测HBoV.
- 分析HBoV基因型及其与临床数据的相关性.
主要方法:
- 在中国天津的不同污水处理站定期采样废水.
- 多重复合实时定量PCR用于HBoV量化.
- 对HBoV DNA序列的遗传学分析.
- 针对病毒检测的安普利康目标测序.
主要成果:
- 在96.6%的废水样本中检测到HBoV.
- HBoV2和HBoV3是主要的基因型.
- 观察到HBoV2和HBoV3丰度的显著季节性变化 (p <0.05).
- 检测到的废水基因型与区域临床数据相关.
- 在废水中发现了HBoV4,但在临床样本中没有发现.
结论:
- 市政废水显示HBoV的高流行率.
- 安普利康的目标测序对于在废水中检测各种病毒,包括新型病毒,是有效的.
- 废水监测可以为有关HBoV循环的公共卫生战略提供信息.
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