废水参数和万科米辛度对抗万科米辛的细菌大肠杆菌及其宿主特异性细菌菌体在医院废水中的溶解活性的影响
P Nithiya1, G Alagarsamy1, P B Sathish1
1Department of Nanobiotechnology, PSG Institute of Advanced Studies, Coimbatore, 641004, India.
Environmental research
|February 5, 2024
概括
菌体可以有效地对抗医院废水中的耐万科米辛大肠杆菌 (VRE). 这项研究证明了菌体疗法.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物技术是生物技术.
背景情况:
- 细菌中万科米辛耐药性是一个全球性的健康问题,在医院环境中越来越普遍.
- 医院的废水是抗生素耐药细菌 (ARB) 的潜在储存库.
- 细菌被探索为一种可持续的生物解决方案来对抗ARB.
研究的目的:
- 在真正的医院废水中,研究菌体治疗对抗抗康迈素耐药大肠杆菌 (VRE) 的疗效.
- 了解废水特性对菌体-VRE相互作用的影响.
- 描述VRE及其特定的菌体.
主要方法:
- 使用16S rRNA测序,MALDI-TOF和全基因组测序来隔离和识别VRE.
- 使用电子显微镜,动态光散射,光谱测量和双甲覆盖的菌体感染性测定.
- 在不同的废水条件下 (pH,温度,万科胺度) 对菌体与宿主相互作用的分析.
主要成果:
- 隔离的菌体在pH 7和37°C时表现出类似T7的形态和最佳溶解.
- 在医院废水中,VRE的最大溶解 (100%) 得到了50μg/mL的vancomycin.
- 菌体活动导致废水中的Ca,K,Mg和P度下降.
- 整个基因组测序确定了mdfA基因,该基因负责万科米辛耐药性,以及在VRE.病毒中赋予重金属耐药性的基因.
结论:
- 菌体治疗是控制医院废水中的VRE的可行策略.
- 废水特性显著影响菌体的疗效,确定了最佳条件.
- 该研究提供了对VRE耐药性的遗传基础及其与复杂环境矩阵中的菌体相互作用的见解.
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