在Atg1/ULK复合物的代谢物传感器子单元调节选择性自
A S Gross1,2, R Ghillebert1, M Schuetter3
1Max Planck Research Group of Autophagy and Cellular Ageing, Max Planck Institute for Biology of Ageing, Cologne, Germany.
Nature cell biology
|February 5, 2024
概括
酸盐饥饿会触发一种独特的自途径. 代谢物传感器Pho81通过与Atg11/FIP200复合体相互作用来调节食,绕过Atg13和Atg17等核心自蛋白.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 代谢过程中的代谢.
背景情况:
- 细胞利用自为应激适应和代谢调节.
- Atg1/ULK激酶复合体 (AKC) 是诱导自的核心.
- 蛋白激酶网络调节AKC活动,以响应代谢线索.
研究的目的:
- 为了调查代谢物传感器Pho81在酸盐饥饿期间自的作用.
- 为了阐明Pho81调节食的机制.
- 为了探索Atg1/ULK激酶复合物的组成可塑性.
主要方法:
- 研究了Atg1/ULK激酶复合体中的蛋白质-蛋白质相互作用.
- 分析了Pho81,Atg11和FIP200在酸盐饥饿诱导的自的作用.
- 评估了核心AKC组件 (Atg13,Atg17) 对于这种特定的自反应的必要性.
主要成果:
- Pho81通过一种特定的动机与Atg11/FIP200复合体相互作用,调节食.
- Pho81的SPX域感知到酸盐代谢物.
- 核心AKC组件Atg13和Atg17对于酸盐饥饿诱导的自不可少.
- 81通过Atg1促进Atg11的酸化,补偿Atg13的不活性.
结论:
- Pho81充当了对AKC的代谢信息通道,独立于正规AKC组件调节食.
- 自调节表现出显著的组成和功能性可塑性.
- 像Atg11/FIP200这样的适配器子单元可以结合选择性自受体和代谢调节器.
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