历史性甲素耐药黄金葡萄球菌:使用瑞士遗留收藏的分子流行病学表征来扩大当前的知识
Vanni Benvenga1,2, Aline Cuénod1,2, Srinithi Purushothaman1,2
1Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland.
Genome medicine
|February 5, 2024
概括
这项研究描述了早期的甲基西林耐药黄金葡萄球菌 (MRSA) 隔离物,揭示了新的序列类型 (ST) 以及对MRSA演变的见解. 这些发现揭示了瑞士和世界之间的MRSA血统流动,超过五十年.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 流行病学 流行病学
背景情况:
- 对于早期的抗甲素黄金葡萄球菌菌株 (MRSA) 存在有限的基因组数据,这阻碍了对成功血统背后的进化驱动因素的理解.
- 鉴定历史MRSA分离物的特征对于追踪特定多位点序列类型 (MLST) 的出现和传播至关重要.
研究的目的:
- 从1965年至1987年的MRSA分离物的遗产集合的特征.
- 将历史的瑞士MRSA基因组与国际和当代分离物进行比较,以了解进化动态和血统流动.
主要方法:
- 全基因组测序 (WGS) 使用Illumina短读和牛津纳米孔长读技术对451个历史的瑞士MRSA分离物进行测序.
- 表型抗微生物药物耐药性 (AMR) 分析.
- 基因组比较分析包括核心基因组 (cg) MLST,SCCmec类型和AMR和病毒性基因的识别,以及公开和现代的MRSA数据集.
主要成果:
- 在历史隔离物中确定了17种序列类型 (ST),包括5种新型ST (ST7843,ST7844,ST7837,ST7839,ST7842).
- ST247-MRSA-I主导了早期分离物 (1960年代),而ST7844 (ST239的一个变体) 后来出现. 克隆复合体 (CC) 8在早期占主导地位,在20世纪末出现了CC22,CC5和CC8.
- 基因组分析揭示了ST239-MRSA-III.多样性内的新型ST7844分离物的聚类.
结论:
- 该研究在早期的MRSA采集中发现了新的ST,并提供了对MRSA的进化历史和血统交换的见解.
- 分析了超过1700个历史和当代隔离物,跨越50年,阐明了瑞士和国际MRSA种群之间的动态流动.
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