哈普洛型解析的基因组组装提供了对Actinidia arguta tetraploid进化历史的见解
Feng Zhang1, Yingzhen Wang1,2, Yunzhi Lin3
1School of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
Molecular horticulture
|February 5, 2024
概括
耐受性基维果 (Actinidia arguta) 的第一个单 haplotype 解析的四倍体基因组揭示了其多倍体进化的洞察力. 这种基因组组装有助于理解改善水果质量和环境适应性的遗传变异.
科学领域:
- 基因组学就是基因组学.
- 植物科学 植物科学
- 进化生物学 进化生物学
背景情况:
- Actinidia arguta,或坚固的,是一种有价值的作物,以其独特的特征而闻名,如小,光滑的水果,营养丰富和耐寒性.
- 这种物种表现出了显著的形多样性,包括双形,四形,六形,八形和十形.
研究的目的:
- 报告了第一个由哈普洛型解析的四体基因组组装Actinidia arguta cv. "长城第二号".
- 分析基因组结构,合成和进化史,包括全基因组复制 (WGD) 事件.
- 研究与免疫反应,环境适应性和水果成熟相关的遗传变异.
主要方法:
- 哈普洛型解决的基因组测序和组装一个四分体的Actinidia arguta个体.
- 阶段性基因组结构,合成和进化分析,包括基因对的Ks计算.
- 标注特定基因家族 (NBS-LRRs,CBFs) 和加权基因同表达网络分析 (WGCNA) 的收获后质量指标.
主要成果:
- 组装了一种带有四个类型和详细基因计数的单 haplotype 解析的四平分体基因组.
- 整个基因组复制 (WGD) 事件的日期是,四化估计约为1.03亿年前,早期的Ad-α事件约为18.7亿年前.
- 确定了参与免疫反应,耐寒性和果实成熟调节的关键基因,突出显示了多化后遗传变异的作用.
结论:
- 在Actinidia arguta四体基因组的组装提供了一个有价值的基因组资源.
- 这一资源将促进进一步的功能基因组学研究和对固基维果的基因改进策略.
- 了解复杂的基因组结构和进化历史可以为增强作物特征的育种努力提供信息.
更多相关视频
10:40Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
10.5K
13:03Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
10.6K
相关概念视频
Genome Annotation and Assembly
18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K
Evolutionary Relationships through Genome Comparisons
5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.7K
Synteny and Evolution
3.3K
John H. Renwick first coined the term “synteny” in 1971, which refers to the genes present on the same chromosomes, even if they are not genetically linked. The species with common ancestry tend to show conserved syntenic regions. Therefore, the concept of synteny is nowadays used to describe the evolutionary relationship between species.
Around 80 million years ago, the human and mice lineages diverged from the common ancestor. During the course of evolution, the ancestral...
Around 80 million years ago, the human and mice lineages diverged from the common ancestor. During the course of evolution, the ancestral...
3.3K
Introduction to Actin
5.2K
Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution. Actin coding genes are conserved within species and across...
5.2K
Polytene Chromosomes
10.0K
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
10.0K
Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K
