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Updated: Jul 4, 2025

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
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在大肠杆菌中控制的蛋白质降解:新的方法和参数

Glen E Cronan1, Andrei Kuzminov1

  • 1Department of Microbiology, University of Illinois at Urbana─Champaign, Urbana, Illinois 61801, United States.

ACS synthetic biology
|February 6, 2024
PubMed
概括

研究人员使用SspB.在大肠杆菌中开发了一种新的可诱导蛋白质降解系统. 虽然对某些蛋白质有效,但基质特异性限制和新型蛋白酶抗性机制阻碍了这种降解工具的广泛采用.

关键词:
在ClpXP蛋白酶中.这里是ReCAA.在RuvBB上,你会看到RuvB.这是一个SspB适配器.降级 degron 降级 降低 降低

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