相关实验视频

Updated: Jul 4, 2025

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

外基因组:在微生物感染中是朋友还是敌人?

Samane Teymouri¹, Maryam Pourhajibagher², Abbas Bahador^1,3

¹Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Infectious disorders drug targets

|February 6, 2024

概括

抗微生物光动力疗法 (aPDT) 提供了一种针对耐药性感染的新策略. 外体显示出作为aPDT的药物载体的希望,提高治疗效率并克服传统光敏剂的局限性.

科学领域:

微生物学微生物学
生物技术是生物技术.
照相医疗 (photomedicine) 是一种医学.

背景情况:

耐药性病原体对全球健康构成重大威胁.
抗微生物光动力疗法 (aPDT) 是传统抗生素的一个有希望的替代品,没有报告的耐药性.
在aPDT中使用的光敏感剂 (PS) 往往具有不良的稳定性和生物可用性,需要有效的药物输送系统.

研究的目的:

审查外体的潜力作为抗菌光动力学治疗 (aPDT) 的药物递送平台.
突出外体在克服光敏剂的局限性方面的优势.
探索外体在对抗微生物感染中的新兴作用.

主要方法:

文献综述侧重于抗微生物光动力学疗法 (aPDT) 和基于外体的药物输送.
对aPDT的机制进行分析,涉及光敏化剂,光和氧气,以产生活性氧物种 (ROS).
对外体特征的评估,包括低免疫性,稳定性和细胞透性,用于药物输送应用.

主要成果:

外体体显示出作为光敏感体的载体的潜力,提高了它们的稳定性,溶解性和生物可用性.
外基因组具有固有的抗微生物特性,尽管它们在病原性中的作用需要进一步调查.
外体和aPDT的组合提供了对抗微生物感染的协同方法.

结论:

外体体代表了一个有前途的纳米载体平台,可以提高抗菌光动力学治疗 (aPDT) 的有效性.
对外体介导药物递送的进一步研究对于开发抗药性感染的新策略至关重要.
外体提供了多方面的方法,将药物输送和潜在的抗菌活性结合起来,用于未来的治疗应用.

关键词:

外基因组是外基因组的组成部分.抗微生物光动力学疗法.药物输送是药物输送的过程.细胞外囊泡中的细胞外囊泡.微生物感染微生物感染活性氧物种. 活性氧物种.

更多相关视频

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Published on: August 26, 2021

相关概念视频

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

Antimicrobial Proteins

Antimicrobial Proteins

Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...

Surface Membrane Barriers

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...

Bacterial Signaling

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...

Defense Against Bacterial Pathogens

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...