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在暴露于阳台的阳台的紫外线辐射后,在小鼠皮肤中的转录组分析

Sami S Qutob¹, Samantha P M Roesch¹, Sandy Smiley¹

¹Consumer and Clinical Radiation Protection Bureau, Health Canada, Ottawa, Ontario, Canada.

Photochemistry and photobiology

|February 6, 2024

概括

紫外线 (UV-R) 暴露会改变基因表达,增加皮肤癌风险. 这项研究揭示了剂量和时间依赖的基因组变化,为UV-R化提供了洞察力.

科学领域:

基因组学就是基因组学.
皮肤病学皮肤病学
分子生物学分子生物学

背景情况:

紫外线 (UV-R) 暴露,从晒,通过分子和细胞的变化提高皮肤癌症的风险.
现有的关于UV-R诱导的基因组变化的知识仍然不完整,特别是在剂量和时间依赖方面.

研究的目的:

机械地研究由UV-R暴露引起的剂量和时间依赖的基因表达改变.
阐明UV-R晒导致的短期 (如日晒) 和长期 (如皮肤癌) 后果的分子途径.

主要方法:

对C57BL/6N小鼠皮肤的转录组分析,该皮肤在暴露于不同红色加权的紫外线剂量 (040mJ/cm2) 后.
在UV-R暴露后的多个时间点 (6,48,96小时) 进行基因表达分析.

主要成果:

96小时的低紫外线剂量 (5mJ/cm2) 诱导了116个与结构损伤相关的差异表达基因 (DEGs).
在较高剂量 (20和40mJ/cm2) 的情况下,在6小时和48小时观察到最多的DEG.
对于皮肤屏障稳定至关重要的13个DEG在所有时间点都在40mJ/cm2的持续变化,表明包括氧化应激,P53信号和炎症在内的重要途径激活.

结论:

紫外线暴露会在小鼠皮肤中引发显著的剂量和时间依赖的转录基因变化.
这些变化影响关键路径,如氧化应激,DNA损伤反应,炎症和皮肤屏障功能.
这些发现为与UV-R晒相关的短期和长期健康风险提供了机制性的洞察力,表明了潜在的非值效应.

关键词:

造成的DNA损伤是DNA损伤.剂量依赖的变化在活体中,活体中氧化应激是一种氧化应激.皮肤癌是皮肤癌.一个阳台的阳台.转录变化的变化.紫外线辐射是一种紫外线辐射.

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