通过NF-κB信号通路,CGA可以缓解LPS诱导的炎症和BMEC中的牛奶脂肪减少
Chen-Chen Lyu1, Xing-Yu Ji1, Hao-Yu Che1
1College of Animal Science, College of Animal Sciences, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun, Jilin, PR China.
Heliyon
|February 6, 2024
概括
酸 (CGA) 通过减少炎症和氧化应激,有效地对抗奶牛的乳腺炎. 这种天然化合物有助于恢复牛乳腺上皮细胞 (BMEC) 中的牛奶脂肪合成.
科学领域:
- 兽医科学 兽医科学 兽医科学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 乳腺炎显著影响奶牛的牛奶产量和质量.
- 在Eucommia ulmoides叶中发现的酸 (CGA) 具有抗炎和抗氧化特性.
- 了解CGA在细胞水平上对乳腺炎的影响对于乳制品动物健康至关重要.
研究的目的:
- 研究CGA在减轻牛乳腺上皮细胞 (BMECs) 中的脂聚糖 (LPS) 诱导的炎症和牛乳脂减少方面的疗效.
- 阐明CGA作用的潜在分子机制,特别是其对炎症和氧化应激途径的影响.
主要方法:
- 牛乳腺上皮细胞 (BMEC) 用10μg/mL的脂多糖 (LPS) 进行治疗,以诱导乳腺炎模型.
- 使用定量逆转录聚合酶链反应 (QRT-PCR) 和西区分法来评估基因和蛋白质的表达.
- 油红色O染色和甘油三 (TG) 试验被用于评估牛奶脂肪合成.
主要成果:
- 在LPS诱导的BMEC中,CGA治疗显著降低了促炎标记物 (IL-6,IL-8,TNF-α,IL-1β,iNOS) 的表达.
- CGA增加了与抗氧化剂相关的蛋白质 (CHOP,XCT,NRF2,HO-1) 的表达,从而降低了氧化应激.
- CGA缓解了由LPS暴露引起的乳脂合成和甘油三含量减少.
结论:
- 酸 (CGA) 在牛乳腺炎的细胞模型中显示出强大的抗炎和抗氧化作用.
- 通过调节NF-κB信号通路,包括调节P65酸化,CGA可以发挥其保护作用.
- 在乳牛中,CGA在恢复牛奶脂肪含量和减轻乳腺炎相关的细胞损伤方面表现有前途.
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