电磁场对可可发酵中的微生物生长的影响:使用已建立的生长模型进行控制的实验方法
Tania María Guzmán-Armenteros1,2, José Villacís-Chiriboga1, Luis Santiago Guerra3
1Departamento de Ciencia de Alimentos y Biotecnología (DECAB), Escuela Politécnica Nacional (EPN), Quito, Ecuador.
Heliyon
|February 6, 2024
概括
振荡磁场 (OMF) 通过减缓微生物生长来影响可可豆发酵. 这项研究发现,OMF暴露会降低乳酸细菌,酸细菌和酵母菌的生长率,从而影响发酵动力学.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 生物物理学的生物物理.
背景情况:
- 优化可可豆发酵是提高感官和营养素质的关键.
- 电磁场 (EMF) 为精确控制发酵条件提供了一个潜在的方法.
- 了解电磁场对微生物动力学的影响对于过程优化至关重要.
研究的目的:
- 评估振荡磁场 (OMF) 对收集卡斯特罗·纳拉尼亚尔 (CCN 51) 可可豆中的关键微生物群体生长动力学的影响.
- 确定不同生长模型 (Gompertz,Baranyi,Logistic) 适合于描述微生物对OMF的反应.
主要方法:
- 使用赫尔姆霍尔茨线圈,可可豆暴露在不同强度的OMF (0-80mT) 中1小时.
- 乳酸细菌 (LAB),酸细菌 (AAB) 和酵母 (Y) 的活跃种群通过殖民地形成单位 (CFU) 计数量化.
- 使用Gompertz,Baranyi和Logistic模型分析了微生物生长数据.
主要成果:
- 后勤模型准确地描述了OMF下的LAB和酵母生长,而Baranyi模型适合AAB的生长.
- 与对照组相比,OMF暴露导致所有微生物群体的最大特异生长率 (μmax) 较低.
- 酸细菌在5mT时显示出最高的平均生长率,酵母在80mT时显示出最低的平均生长率.
结论:
- 振荡磁场显著影响可可豆发酵微生物的生长动力学.
- 暴露于OMF导致微生物生长率降低和延长滞后阶段,表明其控制发酵的潜力.
- 特定的微生物群体对OMF表现出不同的反应,这表明可可在可可发酵中有针对性的应用.
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