粉蛋白分离物 (Cyamopsis tetragonoloba) 的物理化学,结构和功能特征
Bhavya Kotnala1,2, Vijayaraj Panneerselvam1,2, Arun Kumar Vijayakumar3,2,4
1Department of Biochemistry, CSIR - Central Food Technological Research Institute, Mysore, 570020, India.
Heliyon
|February 6, 2024
概括
瓜尔科尔马蛋白质分离提供高营养质量和优良的功能性质. 这种植物蛋白在食品工业中显示出作为可行的替代品的潜力.
科学领域:
- 食品科学与技术 食品科学与技术
- 植物蛋白研究 植物蛋白研究
- 营养生物化学 营养生物化学
背景情况:
- 食品行业寻求可持续和高质量的植物性蛋白质来源.
- 瓜尔科尔玛和沙里是瓜尔加工中未得到充分利用的副产品.
- 评估它们的蛋白质分离物对于食品应用至关重要.
研究的目的:
- 为了评估 guar korma 蛋白质分离的物理化学,营养,功能,结构和消化性质.
- 为了确定其作为食品成分的适用性.
- 为了将其特性与大豆蛋白分离物进行比较.
主要方法:
- 使用提取水的蛋白质隔离.
- 分析蛋白质含量,产量,氨基酸概况和蛋白质质量指数.
- 通过SDS-PAGE进行蛋白质分析.
- 在体外消化性测试.
- 功能性质评估 (发泡,保持水/油,乳化).
- 使用FTIR,CD光谱和SEM进行结构分析.
主要成果:
- 瓜尔·科尔马蛋白质分离物呈现出89.7%的蛋白质含量和48.7%的产量.
- 证明了优良的蛋白质质量,含有高必需氨基酸含量 (34.35%).
- 与大豆蛋白分离物具有相似的功能性质,包括发泡,保持水/油和乳化稳定性.
- 在15分钟内达到完全的体外消化能力.
- 结构分析揭示了特征性的蛋白质构造和形态.
结论:
- 从水中提取的 guar korma 蛋白质分离物具有优越的营养和功能属性.
- 它是一种高质量的蛋白质来源,富含必需氨基酸.
- 它的特性使其成为食品工业有前途的植物性替代品.
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