抗原静止和呼吸道化应激在人类的初级状腺功能障碍
Benjamin Gaston1, Laura A Smith1, Michael D Davis1
1Herman B. Wells Center for Pediatric Research, Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, Indiana, United States.
初级状动力障碍 (PCD) 损害了抗原清除,导致呼吸道氧化和化应激. 这表明来自抗原静止的氧化是PCD的潜在治疗点.
科学领域:
- 肺部医学 肺部医学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 鼻氧化 (nNO) 水平通常在原发性纤毛功能障碍 (PCD) 患者中降低.
- 降低PCD中的状功能可能会导致抗原积累,增加气道氧化剂的产生和氧化氧化 (NO).
- 这一过程可以减少气相NO,并提高化应激.
研究的目的:
- 研究来自健康对照组 (HCs) 和患有PCD的患者的初级气道上皮细胞中的抗原清除,氧化应激和NO代谢.
- 为了在HC和PCD患者之间比较nNO和呼气凝结物 (EBC) 过氧化 (H2O2) 在体内水平.
主要方法:
- 来自HC和PCD患者的初级呼吸道上皮细胞被用于测量抗原清除,3-尼托铁素 (3-NT) 水平和氧化酶表达.
- 在PCD引起基因型的和没有PCD引起基因型的细胞上进行了头部空间NO测量.
- 在体内测量包括nNO和EBC H2O2.2.
主要成果:
- 与HC细胞相比,PCD细胞中的抗原 (Derp1-f) 清除效率较低.
- 在PCD细胞中,DUOX1的表达被改变了.
- 在PCD细胞中,3-NT和酸盐含量较高,表明化应激增加.
- PCD细胞比HC细胞消耗更多的NO,这种效应被超氧化物脱酶 (SOD) 和apocynin减轻.
- 在体内,nNO较低,而EBC H2O2在PCD患者中高于HC患者.
结论:
- 在PCD患者的呼吸道上皮表上显示出抗原清除受损,氧化和化应激增加.
- 由抗原静止引起的氧化是PCD的潜在治疗点.
- 有用于监测与氧化相关的治疗干预措施的生物标志物.
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