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在肉食动物体内发生性交互蛋白的积极选择
Francisco Pisciottano1, María Clara Campos1, Clementina Penna1
1Instituto de Biología y Medicina Experimental (IByME-CONICET), Buenos Aires, Argentina.
Molecular ecology
|February 6, 2024
概括
肉食动物的繁殖蛋白质进化揭示了Feliformia中较低的多样性. 阳性选择在 zona pellucida (ZP) 蛋白质和IZUMO1中,但不是JUNO,提供了对配体相互作用的见解.
科学领域:
- 分子进化分子进化
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
背景情况:
- 在Pantherinae中,物种间的生殖隔离障碍很弱,因此需要对生殖蛋白进行进化研究.
- 透明区 (ZP) 蛋白质和IZUMO1-JUNO融合对对子体相互作用至关重要.
- 之前的研究还没有广泛探索这些蛋白质在肉食动物阶层的分子进化.
研究的目的:
- 为了研究 zona pellucida (ZP) 蛋白质和肉食动物中 IZUMO1-JUNO 配体融合对的分子进化.
- 为了比较Feliformia和Caniformia分类之间的进化模式,专注于Pantherinae.
- 为了确定特定的进化力量,如积极选择,作用于这些生殖蛋白质.
主要方法:
- 利用染色体解析的美洲虎基因组组件获得编码序列用于进化分析.
- 分析了30多种食肉动物物种的序列变异性和分子进化.
- 应用了植物遗传学方法来重建进化历史,并检测积极选择的特征.
主要成果:
- 与Caniformia相比,Feliformia表现出明显较低的序列多样性.
- 在Feliformia,Caniformia和Pantherinae的ZP2和ZP3中确定了积极选择,但与精子结合无关.
- IZUMO1在基底食肉动物和Caniformia系中表现出强烈的阳性选择,而JUNO在基底食肉动物和Caniformia系中表现出没有阳性选择. 在IZUMO1中,在JUNO交互集群中发现了8个特定于Caniformia的阳性选择站点.
结论:
- 这项研究为ZP蛋白和Carnivora中的IZUMO1-JUNO对的进化轨迹提供了新的见解.
- 不同的进化压力,包括积极选择,塑造了这些生殖蛋白质.
- 这些发现有助于理解食肉动物生殖隔离和物种化的分子基础.
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