在lncRNA20718-miR6022-RLPs模块调节番茄的耐药性,以Phytophthora感染者
Jia-Hui Zhang1, Hong-Bo Wei1, Yu-Hui Hong2
1School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.
Plant cell reports
|February 6, 2024
概括
番茄长非编码RNA 20718通过模仿Sl-miR6022,调节SlRLP6/10表达来增强对Phytophthora infestans的抵抗力. 这项研究揭示了提高番茄病耐药性的关键分子机制.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物病理学 植物病理学
- 遗传学 遗传学 是一个
背景情况:
- 番茄 (Solanum lycopersicum) 容易受到Phytophthora infestans的感染,这会影响产量和质量.
- 长非编码RNAs (lncRNAs) 和microRNAs (miRNAs) 在植物防御机制中至关重要.
- 以前的预测表明Sl-lncRNA20718和Sl-miR6022参与番茄耐药性.
研究的目的:
- 为了阐明Sl-lncRNA20718和Sl-miR6022在番茄对P. infestans.抗性的相互作用.
- 确定Sl-lncRNA20718和Sl-miR6022在番茄防御通路中的调节作用.
- 确定下游目标和参与这种抵抗的分子机制.
主要方法:
- 功能增益和丧失实验以评估Sl-lncRNA20718和Sl-miR6022的作用.
- 针对位点的突变发生证实了Sl-lncRNA20718和Sl-miR6022之间的相互作用.
- 对 SlRLP6/10,PRs 和植物激素 (JA,ET) 的基因表达分析.
- 测量反应性氧物种 (ROS) 积累和损伤直径.
主要成果:
- Sl-lncRNA20718充当正调节剂,而Sl-miR6022充当番茄对P. infestans.耐药性的负调节剂.
- Sl-lncRNA20718 作为 Sl-miR6022 的内源性标模仿 (eTM) 功能,减少其表达.
- Sl-miR6022的目标和分裂SlRLP6/10,影响PRs,ROS,JA和ET等级.
- 过度表达Sl-lncRNA20718增强了抗病能力,而过度表达Sl-miR6022则抑制了它.
结论:
- 该lncRNA20718-miR6022-RLPs模块是番茄对P. infestans.耐药性的关键调节器.
- 该模块影响与疾病抵抗相关的基因表达,ROS积累和植物激素水平.
- 这些发现为开发新的番茄病耐药性育种策略提供了基础.
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