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用scRNA-seqq进行人头皮组织优化解离的协议,用于毛囊转录组学

Zishuo Yuan1, Junjie Chen2, Yanwen Xu3

  • 1BGI Research, Hangzhou 310030, China; BGI Research, Shenzhen 518083, China.

STAR protocols
|February 6, 2024
PubMed
概括

我们优化了人类毛囊单细胞RNA测序 (scRNA-seq) 的协议. 这种方法可确保从头皮组织中提取高质量的单细胞悬浮液,用于转录基因分析.

关键词:
细胞生物学 细胞生物学基于细胞的测试遗传学 遗传学 是一个基因组学就是基因组学.分子生物学分子生物学一个单细胞的单细胞.

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...