MdWER与MdERF109和MdJAZ2相互作用,以调节果果中的甲基斯酸盐和光诱导的安托西亚尼生物合成
Xi Zhang1,2, Lujia Yu1,2, Mengjiao Zhang1,2
1Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing, China.
The Plant journal : for cell and molecular biology
|February 6, 2024
概括
果水果的色彩涉及安托氨酸生物合成,由光和甲基莉酸盐 (MeJA) 调节. 一个关键模块,MdERF109-MdWER-MdJAZ2,协调这些信号以增强水果色素.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 生物化学 生化学
背景情况:
- 果 (Malus domestica) 中的安托西亚宁生产会影响水果的颜色.
- 连接光,甲基斯酸盐 (MeJA) 和安托氨酸生物合成的调节机制尚未完全理解.
- 转录因子 (TFs) 在调解这些途径方面发挥着至关重要的作用.
研究的目的:
- 研究转录因子MdERF109与其他蛋白质在调节果颜色的协同作用.
- 阐明参与MeJA和辐射诱导的氨酸生物合成的调节性相互关系.
- 确定控制果色素的新型成分和机制.
主要方法:
- 酵母两杂交测试以确定蛋白质与蛋白质之间的相互作用.
- 在果果中的基因过度表达和共同表达研究.
- 在果果中进行过渡性共同表达和蛋白质相互作用测试.
- 对安托西亚宁积累和基因表达的分析.
主要成果:
- 确定MdERF109和MdWER是参与氨酸生物合成的相互作用TF.
- 与单独使用MdWER相比,MdERF109和MdWER的同时表达显著增加了安托西亚宁积累.
- MdWER与抑制器MdJAZ2相互作用,表明一个复杂的调节网络.
- 验证了MdERF109-MdWER-MdJAZ2模块对于MeJA和辐射治疗下的安托素生产至关重要.
结论:
- 一个新型的MdERF109-MdWER-MdJAZ2调节模块控制了果中的安托西亚宁生物合成.
- 该模块集成了光和MeJA信号通路,以调节水果颜色.
- 这些发现为控制果色素的分子机制提供了新的见解.
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