提取pH对氨基酸的影响,营养,体外蛋白质消化能力,分子间相互作用,以及瓜尔胚芽蛋白的功能性质
Ankan Kheto1, Rachna Sehrawat1, Khalid Gul1
1Department of Food Process Engineering, National Institute of Technology, Rourkela, Odisha 769008, India.
Food chemistry
|February 6, 2024
概括
瓜尔胚芽蛋白 (GGP) 提取pH影响营养和功能性质. 在pH 9下,GGP的最佳隔离以获得营养益处,观察到最高的蛋白质质量.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物化学 生物化学
背景情况:
- 瓜尔胚芽蛋白 (GGP) 是一种植物性蛋白质的潜在来源.
- 了解提取条件对GGP特性的影响对于其应用至关重要.
研究的目的:
- 研究提取pH对瓜尔胚芽蛋白 (GGP) 的化学组成,分子间相互作用和功能性质的影响.
- 确定最佳的pH值,以隔离具有理想营养和功能特征的GGP.
主要方法:
- 在不同的pH值 (7-11) 提取了瓜尔胚芽蛋白.
- 评估的营养指数 (蛋白质效率比,必需氨基酸指数,预测的生物价值,营养指数,蛋白质纯度).
- 评估了体外蛋白质消化性,SDS-PAGE,颗粒大小,分子间键强度 (离子,),β片含量和变质温度.
主要成果:
- pH 9产生了最高的蛋白质效率比率,必需氨基酸指数,预测的生物价值,营养指数和蛋白质纯度.
- 在体外蛋白质的消化能力在pH11时是最高的.
- SDS-PAGE显示了带强度 (<10 kDa) 与pH的变化.
- pH 11 呈现出最小的粒子大小和最弱的分子间键.
- 在pH 7提取的GGP表现出优越的功能性质.
- 主要成分分析表明pH 9是GGP隔离的最佳值.
结论:
- 提取pH显著影响 guar胚芽蛋白的营养和功能性质.
- 建议使用pH 9来分离具有最高营养质量的GGP.
- 进一步的研究可能会探索针对特定应用的不同pH值的特定功能性质.
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