相关实验视频

Updated: Jul 4, 2025

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

费里结合和无化修饰的mRNA疫苗诱导强烈的免疫反应和对SARS-CoV-2的保护疗效

Tong Yu¹, ChenChao Zhang², JunHong Xing³

¹College of Veterinary Medicine, Jilin Agricultural University, Changchun, China; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China.

International immunopharmacology

|February 6, 2024

概括

新的mRNA疫苗候选人针对SARS-CoV-2变种显示出有希望. 在小鼠和仓鼠的研究显示强大的抗体和细胞免疫反应,显著减少肺病理和病毒载荷的潜在的广泛的传染病应用.

科学领域:

* 疫苗学和免疫学
* 传染病研究传染病研究
* 分子生物学 * 分子生物学

背景情况:

* 严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 持续演变,由于免疫逃生机制,构成重大威胁.
*开发新型和有效的mRNA疫苗对于对抗新出现的SARS-CoV-2菌株和其他传染病原体至关重要.

研究的目的:

*合成和评估六种不同的mRNA疫苗配方,用于抗击SARS-CoV-2.
* 在临床前模型中评估这些新型候选疫苗的免疫性和保护性疗效.

主要方法:

* 合成了六种mRNA疫苗配方:受体结合域 (RBD) 单体,RBD双体 (2RBD),RBD-费里丁 (RBD-Fe),野生型核体 (WT-N),重新排列的核体 (Re-N) 和基于表位素的疫苗,所有这些都被封装在SM102脂质纳米粒子中.
* 在C57BL/6小鼠和黄金仓鼠中进行了免疫研究.
*分析了幽默和细胞免疫反应,以及测量病毒载量,肺病理和细胞因子表达的保护疗效测定.

主要成果:

* RBD单体,2RBD和RBD-Fe疫苗在小鼠中诱导了强大的特定抗体标位,包括中和抗体.
* WT-N,Re-N和以表位基的疫苗主要引起强大的细胞免疫反应.
* 接种疫苗的仓鼠表现出肺部病理的减少,呼吸道组织中的病毒载荷较低,以及炎症性细胞因子水平的降低.

结论:

*开发的mRNA疫苗候选人在临床前模型中显示出显著的免疫性和对SARS-CoV-2的保护性有效性.
*这些发现支持针对新兴病原体的新型疫苗开发策略,并为先进的疫苗配方提供基础.

关键词:

炎症性细胞因子这就是SARS-CoV-2病毒.乌比基化修饰的修饰方法在mRNA疫苗的使用中.

更多相关视频

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Published on: August 17, 2022

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Published on: June 10, 2022

相关概念视频

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...

Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

Cross-reactivity

Cross-reactivity

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Vaccinations

Vaccinations