合成NLR RGA5 HMA5 需要在集成域内外的多个接口来实现效应器识别
Xin Zhang1,2, Yang Liu1,3, Guixin Yuan1,2
1The State Key Laboratory of Maize Bio-breeding, Joint International Research Laboratory of Crop Molecular Breeding, Ministry of Agriculture Key Laboratory for Crop Pest Monitoring and Green Control, College of Plant Protection, China Agricultural University, 100193, Beijing, China.
Nature communications
|February 6, 2024
概括
像米RGA5这样的植物NLR受体使用多个相互作用表面来检测病原体效应物并激活防御. 改造的RGA5变种突显了为植物免疫力采取协调行动的必要性.
科学领域:
- 植物免疫力 植物免疫力
- 分子植物病原体相互作用
- 结构生物学是结构生物学.
背景情况:
- 植物核酸结合氨酸丰富的重复 (NLR) 受体对于检测病原体效应因子至关重要.
- 米RGA5传感器NLR通过其重金属相关 (HMA) 集成域 (ID) 识别Magnaporthe oryzae效应器,激活RGA4助手NLR依赖的电阻.
- 了解NLR受体激活机制是培养作物抗病能力的关键.
研究的目的:
- 调查多个相互作用表面在米RGA5传感器NLR的功能中的作用.
- 为了确定RGA5中的工程接口是否可以对非相应的效应器产生阻力.
- 阐明效应器识别和辅助NLR激活的结构要求.
主要方法:
- RGA5突变的生成和表征,包括具有工程接口的RGA5HMA5.
- 对表达RGA5变异的转基因大米植物对Magnaporthe oryzae菌株的耐药性进行测试.
- 评估使用RGA5变体在米原生质中RGA4依赖的细胞死亡激活.
主要成果:
- RGA5HMA5突变对表达非相应的效应因子AVR-PikD的M. oryzae菌株产生了完全的耐药性.
- RGA5HMA5具有三个工程接口,两个在HMA ID内,一个在C端的Lys丰富的延伸中.
- 只有一个或两个工程接口的RGA5变体未能激活RGA4依赖细胞死亡.
结论:
- 传感器NLRs需要来自多个表面的协调行动,无论是在集成领域内还是外部,以进行效应器识别和辅助NLR激活.
- 这些发现对新型传感器NLR的结构导向设计具有重要意义,以提高作物抗病能力.
- 这项研究强调了植物免疫受体功能的复杂结构基础.
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