塑体祖先缺乏完整的Entner-Doudoroff通路,限制了植物的糖解和酸通路
Sonia E Evans1, Anya E Franks1, Matthew E Bergman1
1Department of Cell and Systems Biology, University of Toronto, Toronto, ON, M5S 3G5, Canada.
Nature communications
|February 6, 2024
概括
植物中没有Entner-Doudoroff通路,因为它们缺乏必要的EDD基因,而不是 prokaryotes. 这条路径的路径.
科学领域:
- 生物化学 生化学
- 植物生物学 植物生物学
- 代谢途径 代谢途径
背景情况:
- 恩特纳-杜多罗夫 (ED) 途径是糖解的替代代代谢途径.
- 它通过脱水酶 (EDD) 和酶 (EDA) 酶将6-酸 (6-PG) 转化为甘甲基-3-酸盐和酸盐.
研究的目的:
- 调查ED路径在高层植物中的分布和重要性.
- 确定ED途径在真核生物中缺失的进化原因.
主要方法:
- 在原核生物和真核生物中基因分布 (EDD和EDA) 的比较分析.
- 对EDD和二氧酸脱水酶 (DHAD) 酶的结构建模.
- 结合酶测试以确认酶的特异性.
主要成果:
- 由于真核生物中缺少EDD基因,ED路径仅限于 prokaryotes.
- EDD和DHAD酶表现出不同的基质特异性,由不同的结构域解释.
- 虽然EDA基因在植物中发现,但在Viridiplantae中没有ED路径.
结论:
- 在更高的植物中,ED路径在进化过程中不存在,因为它们缺乏特定的EDD酶.
- 从没有EDD的塑体祖先中转移一个乱交的EDA酶解释了这种缺失.
- 植物中EDA基因的零星存在是由于这种酶的杂乱性.
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