质量上的调节,而不是定量上的调节,在芽酵母中塑造了化I的结束
Dunja Celebic1,2, Irem Polat1, Véronique Legros1
1Université Paris Cité, CNRS, Institut Jacques Monod, 75013, Paris, France.
The EMBO journal
|February 6, 2024
概括
线粒体退出涉及显著的蛋白质组变化. 介质期I的退出不同,而循环素依赖性激酶 (Cdk) 酸化保持稳定,与线性分裂不同,表明有质的,而不是定量的调节.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 细胞周期进展依赖于动态蛋白质酸化和脱酸化.
- 线性退出涉及降低循环素依赖激酶 (Cdk) 活动和酸酶激活.
- 介质期需要两次分裂而没有DNA复制,需要不同的调节适应.
研究的目的:
- 为了比较芽酵母在线粒脱离过程中的蛋白质组动力学和半变异I到半变异II过渡.
- 为了研究Cdk活性在调节化过程中基质脱化过程中的作用,我退出.
- 了解中介性退出中的调节的定性与定量性质.
主要方法:
- 全球,时间解析的蛋白组学.
- 蛋白质组景观的比较分析.
- 在变过程中诱导人工Cdk活动下降 I.
主要成果:
- 与线粒离子退出不同的是,Cdk基在半变化I结束时仍然在很大程度上化,而线粒离子退出.
- 与Cdk无关的基因在介质变化I到介质变化II过渡时主要脱.
- 介质分裂I期间的人工Cdk活动减少诱导了有序的基质脱,类似于线粒分裂.
结论:
- 介质I结束时的调节主要是定性,而不是定量.
- 与线粒体退出相比,Cdk活动在线粒体退出中起着不同的作用.
- 蛋白质组景观的调节差异化,以适应变的独特需求.
相关概念视频
Meiosis II
45.7K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
45.7K
Meiosis I
193.6K
Meiosis is a carefully orchestrated set of cell divisions, the goal of which—in humans—is to produce haploid sperm or eggs, each containing half the number of chromosomes present in somatic cells elsewhere in the body. Meiosis I is the first such division, and involves several key steps, among them: condensation of replicated chromosomes in diploid cells; the pairing of homologous chromosomes and their exchange of information; and finally, the separation of homologous chromosomes by...
193.6K
Meiosis vs. Mitosis
55.7K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
55.7K
Yeast Signaling
14.6K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.6K
Cells Coordinate Growth and Proliferation
4.5K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
4.5K
Positive Regulator Molecules
106.1K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
106.1K


