脂质同位素和同位素成像使用纳米喷雾溶解电喷雾电离离结合三重四极质谱学
Miranda R Weigand1, Daisy M Unsihuay Vila1, Manxi Yang1
1Department of Chemistry, Purdue University, 560 Oval Dr., West Lafayette, Indiana 47907, United States.
Analytical chemistry
|February 6, 2024
概括
这项研究介绍了纳米喷雾脱电喷雾电离 (纳米-DESI) 质谱成像 (MSI) 在三重四极仪器上. 这种新的方法增强了生物组织中等离子体和等离子体脂质和eicosanoids的空间映射.
科学领域:
- 生物医学科学 生物医学科学
- 分析化学 分析化学
- 分子成像分子成像技术
背景情况:
- 质谱成像 (MSI) 显示生物样本中的分子分布.
- 传统的MSI很难区分异构和异构物种.
- 高分辨率的质谱仪通常用于同位素分离,但复杂的混合物会带来挑战.
研究的目的:
- 在三重四极 (QqQ) 质谱仪上实施纳米喷雾脱电喷雾电离 (纳米-DESI) MSI.
- 为了使生物组织中异构体和异构体物种的空间映射.
- 克服传统MSI在解决复杂分子混合物的局限性.
主要方法:
- 纳米喷雾脱电喷雾电离 (纳米-DESI) MSI是在三重四极 (QqQ) 质谱仪上进行的.
- 多重反应监测 (MRM) 采集模式使用了单位质量分辨率.
- 在小鼠大脑中的脂质和老鼠脏组织中的eicosanoids被成像.
主要成果:
- 纳米-DESI MSI 平台成功地绘制了异构和异构物种的地图.
- 在MRM模式下,分辨出异基脂,相当于3,800,000的质量分辨能力.
- 以前很难定位的eicosanoid异构体,在老鼠脏组织中成功成像.
结论:
- 在QqQ仪器上的Nano-DESI MSI为分子成像提供了增强的特异性和选择性.
- 这种方法允许空间定位以前未解决的同位体和同位体物种.
- 该平台显著提升了MSI对复杂生物样本的功能.
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