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曼科泽布通过损害氧化酸化途径诱导毒性:一个转录组研究

Yan Zhang¹, Ran Wen¹, Jialu Bao¹

¹College of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China.

Ecotoxicology and environmental safety

|February 7, 2024

概括

曼科泽布 (MCZ) 通过破坏氧化酸化,导致氧化应激增加,在小鼠中引起损伤. 这种机制涉及改变基因表达和关键代谢物,最终损害功能.

科学领域:

毒理学毒理学毒理学
分子生物学分子生物学
生物化学生化学

背景情况:

曼科泽布 (MCZ) 是一种广泛使用的杀菌剂.
了解MCZ诱导的毒性分子机制对于风险评估至关重要.

研究的目的:

为了阐明 mancozeb (MCZ) 诱导的损伤的潜在机制.
研究MCZ暴露对小鼠功能,基因表达和代谢途径的影响.

主要方法:

鼠被暴露在MCZ (100mg/kg) 或对照剂 (脱离离子水) 中30天.
分析了功能指标,氧化应激标志物,转录组和代谢组.
用PCR验证基因表达.

主要成果:

暴露于MCZ会损害脏结构,并显著增加肌素和尿酸水平.
通过增加活性氧物种 (ROS) 和MDA,MCZ诱导了氧化应激,同时减少了SOD和GSH-PX.
转录组分析显示了氧化酸化途径的丰富,具有特定的基因表达变化 (例如,ndufs1,ndufab1的上调;cox5b,ndufa5,ndufa6的下调).
代谢分析确定了cGMP-PKG信号通路以及关素单酸盐和腺素5'-单酸盐的水平变化.

结论:

在小鼠中,MCZ主要通过阻断氧化酸化途径来损害功能.
这种阻塞导致氧化应激增加和随后的损伤.
这些发现提供了对MCZ毒性分子病变的洞察力.

关键词:

脏 - 脏 - 脏曼科泽布 (Mancozeb) 是一个代谢组中的代谢.氧化酸化路径的氧化酸化路径氧化应激是一种氧化应激.转录组转录组就是一个转录组.

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