鱼类特定的IFNγ相关结合与独特的受体复合体的表征,并通过新的途径发出信号
Yasuhiro Shibasaki1, Takeshi Yabu1,2, Hajime Shiba1
1College of Bioresource Sciences, Nihon University, Fujisawa, Japan.
FEBS open bio
|February 7, 2024
概括
鱼类具有独特的干扰素,称为干扰素玛相关 (IFNγrel) 蛋白质. 这项研究揭示了IFNγrel 1如何通过特定的受体发出信号,与其他脊椎动物不同,为鱼类的干扰素进化提供了洞察力.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 鱼类拥有两种II型干扰素:IFNγ和鱼类特定的IFNγ (IFNγrel).
- 两种IFNγrel基因 (IFNγrel 1和IFNγrel 2) 在金布纳十字鱼中表现出抗病毒活性.
- IFNγrel 1促进了异种移植的排斥,但其受体和信号通路仍然未知.
研究的目的:
- 为了阐明IFNγrel 1的信号机制和受体在金布纳鱼.
- 了解IFNγrel 1激活的独特途径.
- 将IFNγrel 1归类为更广泛的干扰素家族.
主要方法:
- 调查了STAT6酸化和转录激活,以应对复合Ginbuna IFNγrel 1 (rgIFNγrel 1) 的反应.
- 使用流细胞计检测rgIFNγrel 1与II类细胞因子受体家族成员 (Crfbs) 结合,特别是Crfb5和Crfb17.
- 采用化学交联检测Crfb5和Crfb17二分化,并测量过度表达细胞中的Isg15基因表达.
主要成果:
- rgIFNγrel 1诱导了鱼细胞中的STAT6酸化和转录激活.
- rgIFNγrel 1与Crfb5和Crfb17结合,在结合时二元化.
- Crfb5和Crfb17的过度表达增强了IFNγrel 1诱导抗病毒基因Isg15.g的表达.
结论:
- IFNγrel 1通过独特的受体对 (Crfb5和Crfb17) 激活STAT6,与IFNγ和其他脊椎动物IFN不同.
- 这种信号级联表明IFNγrel 1代表了脊椎动物中一种新的干扰素家族成员.
- 这些发现有助于理解骨鱼中的干扰素基因演变.
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