光子神经探针启用微内镜用于光片光场计算光脑成像
Peisheng Ding1,2, Hannes Wahn1, Fu-Der Chen1,2,3
1Max Planck Institute of Microstructure Physics, Halle, Germany.
Neurophotonics
|February 7, 2024
概括
我们开发了一种新的微内镜,使用光板神经探针和图像纤维进行深度脑部成像. 该系统能够在体内进行高对比度,体积光成像.
科学领域:
- 神经科学是一个神经科学.
- 光学工程是指光学工程.
- 生物医学成像技术 生物医学成像技术
背景情况:
- 光片光显微镜对于高速,高对比度的体积成像至关重要.
- 传统显微镜在体内对非透明生物进行脑部成像时面临几何限制.
- 可植入的光子神经探针具有小型化的激发光学,用于深层组织成像.
研究的目的:
- 开发一种用于无透镜,光场,深度脑组织的计算光成像的微内镜.
- 将光板神经探针与微型光收集光学集成在一起.
主要方法:
- 制造基于的光板神经探测器,具有五个可定位板.
- 包装探头与商业上可用的图像纤维捆绑,以创建微内镜.
- 用光珠和固定小鼠大脑组织测试微内镜的性能.
主要成果:
- 使用开发的微内镜,证明了体积光片光场光成像.
- 与传统方法相比,实现了更大的成像深度.
- 观察到与照明光场成像相对增强的重建精度.
结论:
- 开发的微内镜为体积大脑组织成像提供了一个紧的,高对比度的解决方案.
- 概念验证演示验证了深度大脑光成像的成像方法.
- 这项工作为未来使用光子神经探针启用微内镜的体内应用奠定了基础.
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