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Updated: Jul 4, 2025

Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions
08:54

Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions

Published on: August 8, 2012

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[矩形水凝微槽的空间约束调节了人静脉内皮细胞的形态和排列]

Wenli Jiang1, Jian Zhong1, Zhi Ouyang1

  • 1（ 610041） Institute of Biomedical Engineering, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China.

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
|February 7, 2024
PubMed
概括

这项研究表明,微尺度的水凝槽有效地引导人静脉内皮细胞 (HUVECs) 的对齐和形态. 这些发现突显了这些工程微环境在研究血管细胞行为方面的潜力.

关键词:
细胞的方向 细胞的方向细胞形态 细胞形态细胞骨架 细胞骨架微型纹 微型纹血管内皮细胞血管内皮细胞

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High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

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Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
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