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LncRNA HCG18影响糖尿病心肌病及其与miR-9-5p/IGF2R轴的关联

Yuhui Luo¹, Yi Jiang², Tingting Zhong¹

¹Department of Cardiology, Chongqing General Hospital, University of Chinese Academy of Sciences, Chongqing, 40013, China.

|February 7, 2024

概括

长非编码RNA HCG18在糖尿病心肌病 (DCM) 中升高. 减少HCG18通过调节miR-9-5p/IGF2R通路来保护心脏细胞,为DCM提供了潜在的治疗标.

科学领域:

心血管生物学心血管生物学
分子医学是分子医学.
内分泌学在内分泌学.

背景情况:

糖尿病心肌病 (DCM) 是糖尿病的一个严重并发症.
导致DCM进展的分子机制尚未完全理解.
长非编码RNAs (lncRNAs) 正在成为心血管疾病的关键调节者.

研究的目的:

研究IncRNA HCG18在DCM病原发生中的作用.
阐明涉及DCM中HCG18的潜在分子机制.
探索HCG18作为DCM的潜在治疗点.

主要方法:

通过使用链毒素 (STZ) 诱导了一种DCM的老鼠模型.
H9c2心肌细胞被培养并用高葡萄糖处理以模仿DCM在体外.
进行了定量实时PCR,TUNEL测定和酶活性测定.

主要成果:

在STZ诱导的DCM大鼠模型和高葡萄糖处理的H9c2细胞中,HCG18表达显著上调.
低调 HCG18 改善了细胞活力,减少了细胞亡,并调节了酶活动.
HCG18调节了miR-9-5p,这反过来又针对IGF2R,揭示了一个HCG18/miR-9-5p/IGF2R轴.

结论:

lncRNA HCG18在糖尿病心肌病的进展中起着至关重要的作用.
下调HCG18通过miR-9-5p/IGF2R通路对心肌细胞损伤产生保护作用.
HCG18代表了管理DCM的有前途的治疗标.

关键词:

糖尿病心肌病糖尿病心肌病在 HCG18 的情况下.在IGF2R中,IGF2R是最常见的.在 miR-9-5p 中.

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