考虑到患者内部的多样性,突出显示了抗菌素耐药性Enterococcus faecium中传播途径和抗菌素耐药性基因的变异性
Martin P McHugh1,2, Kerry A Pettigrew1, Surabhi Taori2
1School of Medicine, University of St Andrews, St Andrews, UK.
The Journal of antimicrobial chemotherapy
|February 7, 2024
概括
患者内部的抗万科胺素的Enterococcus faecium (VREfm) 的多样性可能会影响疫情调查. 测序多个殖民地对于医疗保健机构中准确的VREfm传输网络解析至关重要.
科学领域:
- 临床微生物学 临床微生物学
- 传染病流行病学 传染病流行病学
- 基因组流行病学基因组流行病学
背景情况:
- 全基因组测序 (WGS) 对于跟踪与医疗保健相关的抗万科迈素Enterococcus faecium (VREfm) 疫情至关重要.
- 患者内部的遗传多样性可能会使准确的VREfm传播途径的确定变得复杂.
研究的目的:
- 评估VREfm患者内部多样性对传输网络解决方案的影响.
- 评估WGS在识别VREfm传输事件中的有效性.
主要方法:
- 从直肠屏幕收集了14个殖民地,并从Illumina WGS的临床样本中收集了单个殖民地.
- 利用牛津纳米孔测序来进行混合基因组组合和谱系特定的参考生成.
- 使用与密切相关的引用进行映射,以识别基因变异,并使用Phyloscanner推断传输网络.
主要成果:
- 从11名患者中测序了229个VREfm分离物;27%的患者具有23序列类型 (ST).
- 在患者和STs中观察到抗菌素耐药性基因和等离子体的变异性.
- 在主导性STs (ST80,ST1424) 中确定了传染集群,当测序不到14个殖民地时,分辨率受损.
结论:
- 患者可以携带多个具有不同遗传元素和耐药性基因的VREfm STs.
- 承认VREfm患者内部的多样性对于准确的传输网络分析至关重要.
- 建议对多个殖民地进行测序,以提高VREfm爆发调查的准确性.
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