在eDAH和unjamming中非线性应变强化的影响
Xiaofan Xie1, Frank Sauer1, Steffen Grosser1
1Soft Matter Physics Division, Peter Debye Institute for Soft Matter Physics, University of Leipzig, Germany. jkaes@uni-leipzig.de.
Soft matter
|February 7, 2024
概括
细胞在集群中延长会增加组织表面张力,并导致细胞解. 这项研究使用原子力显微镜揭示了细胞的应变硬化,这是多细胞球形力学的一个关键因素.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 材料科学 材料科学 材料科学
背景情况:
- 细胞群体表现出增强的组织表面张力和细胞解过渡,导致显著的细胞延长.
- 这种实质性的延长超过了线性弹性,需要对细胞机械性质变化的研究.
研究的目的:
- 用原子力显微镜 (AFM) 研究显著细胞延长对单个细胞机械性能的影响.
- 在不同的延长条件下分析细胞球体的表面和体积力学.
主要方法:
- 原子力显微镜 (AFM) 用于分析在延长下单个MCF-10A和MDA-MB-436细胞的机械性能.
- 此外,AFM还在动态频率范围 (1-200 Hz) 的细胞球体上进行了进一步利用,以评估表面和散装力学.
主要成果:
- 两种细胞系MCF-10A和MDA-MB-436都表现出随着延长的增加而变硬.
- 显示强烈收缩组织张力的MCF-10A细胞,表现出更大的表面刚性.
- MDA-MB-436细胞的特征是显著的延长,在体积区域表现出增加的刚性.
结论:
- 单细胞菌株硬化是理解多细胞球体 (MCS) 机制的一个关键因素.
- 这种现象甚至适用于更软的细胞类型,如MDA-MB-436,强调其在细胞力学中的广泛适用性.
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