相关实验视频

Updated: Jul 4, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

关于临床前列腺癌组织的空间MS多组学

Jacob X M Truong^1,2,3,4, Sushma R Rao^1,2, Feargal J Ryan^2,5

¹The University of Adelaide, North Terrace, Adelaide, SA, 5000, Australia.

Analytical and bioanalytical chemistry

|February 7, 2024

概括

这项研究从单个前列腺癌样本中整合了空间脂组学和蛋白质学. 它确定了溶解脂胆酸转移酶1 (LPCAT1) 作为一种在癌症组织中丰富的关键蛋白质标记物.

科学领域:

生物化学生物化学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

质谱学 (MS) 和MS成像学 (MSI) 对脂管学和蛋白质学至关重要,但通常需要单独的样本准备.
奥米克技术和生物信息学的进步使得从单个样本进行多奥米克分析成为可能.

研究的目的:

在同一样前列腺癌组织样本上开发和验证整合空间脂组学和蛋白质学的工作流.
使用多组学方法识别分离癌细胞和良性前列腺组织的分子标记物.

主要方法:

在前列腺切除核心上进行空间脂管学 (spatial lipidomics) 的矩阵辅助激光脱/离子化MS成像 (MALDI-MSI).
激光捕获微解剖 (LCM) 以MSI数据为指导,用于特定区域的蛋白质组学.
使用dia-PASEF工作流程获取数据,并使用SCiLS区域映射软件进行分析.

主要成果:

在切割的组织区域中识别了5525个独特的蛋白质群.
与良性上皮质 (BE) 相比,癌性上皮质 (CE) 中的lysophosphatidylcholine acyltransferase 1 (LPCAT1) 的显著丰富.
在CE中增加LPCAT1蛋白丰度与改变的酸丁 (PC) 和酸丁 (LPC) 离子强度比率的相关性.

结论:

集成的MSI-proteomics工作流成功地描述了癌症和良性前列腺组织之间的分子差异.
LPCAT1是前列腺癌的潜在生物标志物,其丰富程度反映在蛋白质和脂质组两种配置文件中.

关键词:

激光捕捉微解剖是指激光捕捉的微解剖.利皮多米克 (Lipidomics) 是一种消化剂.马尔迪 (Maldi) 的成像技术多种组合的多种组合.前列腺癌是什么意思前列腺癌是什么意思蛋白质组学是指蛋白质组学.

更多相关视频

Author Spotlight: Advancing Prostate Cancer Research Through Improved Tissue Sampling and Biobanking

Author Spotlight: Advancing Prostate Cancer Research Through Improved Tissue Sampling and Biobanking

Published on: November 17, 2023

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

相关概念视频

Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...