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Updated: Jul 4, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

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基于CRISPR/Cas9的工具包用于快速标记物回收和在Komagataella phaffii中的组合图书馆

Wei Zhou1, Yuanyi Li1, Guosong Liu1

  • 1State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B.311, Shanghai, 200237, China.

Applied microbiology and biotechnology
|February 7, 2024
PubMed
概括

这项研究增强了科马加泰拉 (Komagataella phaffii) 酵母中的CRISPR基因编辑. 新的方法使有效的多基因集成,大片段删除和代谢工程的快速库建设成为可能.

关键词:
这就是CRISPR/Cas9的作用.科玛格塔贝拉 (Komagataella phaffii) 是一个有趣的植物.大型DNA片段的淘汰赛.多片段集成多片段集成视觉标记物的回收利用酵母组装了图书馆的酵母.

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