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编码flavonol合成酶的BrFLS基因突变诱导了中国白菜中的安托西亚宁积累

Jiaqi Zou¹, Shengnan Huang¹, Yue Gao¹

¹College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenhe District, Shenyang, 110866, People's Republic of China.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

|February 7, 2024

概括

中国卷心菜中BrFLS基因的突变导致氨酸的积累增加,导致鲜的紫色. 这一发现为修改中国白菜品种提供了一个新的目标,以提高营养和美学价值.

科学领域:

植物遗传学和育种.
生物化学生物化学
农作物科学农作物科学

背景情况:

中国白菜是一种重要的蔬菜作物,由于其营养和美观益处,消费者对富含安东素的品种的需求日益增加.
负责充满活力的颜色的安托西亚宁在粮食作物中越来越受欢迎.
了解抗生素积累的遗传基础是开发改进品种的关键.

研究的目的:

为了识别紫色的中国卷心菜突变体中负责安东素积累的基因.
为了研究底层的分子机制安托素生物合成调节.
为繁殖多彩的中国白菜提供遗传点.

主要方法:

突变查和特征在中国白菜中一个安东积累突变 (aam1).
进行MutMap映射和KASP基因定型,以确定候选因果基因 (BraA10g030950.3C).
基因表达分析,酶活性测定和代谢物分析.

主要成果:

一个衰退的核基因,BraA10g030950.3C,编码黄醇合成酶 (BrFLS),被确定为因果基因的安东积累.
BrFLS中的突变导致了黄醇合成酶活性的降低和氨酸生物合成的增加.
BrFLS的表达减少了,而BrDFR的表达在突变者中增加了,转移了代谢流向酸盐.

结论:

BrFLS基因的突变是中国卷心菜中安托素积累的关键驱动因素.
这项研究确定了BrFLS作为基因改造的关键目标,以增强中国白菜的色彩.
这些发现有助于开发出新的,视觉上有吸引力的,营养上增强的中国白菜品种.

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