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使用基于SiR-DNA的流细胞计学培养和功能性操纵平面神经母细胞的协议.

Wenya Zhang1, Xinran Li2, Yun Zhao3

  • 1Westlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Growth Regulation and Translational Research of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China; Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310024, China; School of Basic Medical Sciences, Fudan University, Shanghai 200030, China.

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概括

这项研究引入了一种培养和操纵平面神经质细胞的新方法,即负责再生的干细胞. 新的SiR-DNA流式细胞计量协议避免了细胞循环的抑制,使得对神经细胞多能性和再生进行更好的研究.

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细胞培养培养的细胞培养.细胞隔离细胞的隔离.发育生物学是发展生物学.模型生物模型生物干细胞是干细胞.

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科学领域:

  • * 发育生物学 发育生物学
  • * * 干细胞生物学
  • * 分子生物学 * 分子生物学

背景情况:

  • *新芽细胞是平面动物中唯一的增殖细胞,对于再生至关重要.
  • * 传统的基于Hoechst的流细胞计抑制细胞循环,限制了新芽细胞研究.
  • *需要一种非抑制的方法来研究神经细胞功能和多能性.

研究的目的:

  • * 提出一种用于培养和功能性操纵平面神经质细胞的新方案.
  • *使用基于SiR-DNA的流细胞计来进行非抑制性新芽细胞分析.
  • * 为了进一步研究平面干细胞多能性和再生机制.

主要方法:

  • * 开发一种基于SiR-DNA的流细胞测量协议,用于平面神经母细胞.
  • * 细胞解离,染色和流细胞计的详细步骤.
  • * 新芽细胞采集,培养和纳米化酶mRNA转染的程序.

主要成果:

  • *成功地在没有细胞循环抑制的情况下对平面神经质细胞进行SiR-DNA流细胞计.
  • * 建立一个培养和功能性操纵新芽细胞的协议.
  • * 在功能研究中证明mRNA转染的可行性.

结论:

  • * SiR-DNA流式细胞计量协议为研究平面神经母细胞提供了一种非抑制的方法.
  • *本协议提高了研究神经细胞多能性和再生的能力.
  • * 促进对平面再生背后的分子机制的未来研究.