相关实验视频

Updated: Jul 4, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

通过CLiPPR-Seq对mRNA相互作用的圆形RNA进行全球识别

Suman Singh^1,2, Sharmishtha Shyamal¹, Arundhati Das¹

¹Institute of Life Sciences, Nalco Square, Bhubaneswar, Odisha 751023, India.

Nucleic acids research

|February 7, 2024

概括

研究人员开发了一种新方法,即交叉链接多A拉向RNase R测序 (CLiPPR-seq),以发现与信使RNA (mRNA) 相互作用的循环RNA (circRNA). 这种技术揭示了circRNAs如何调节基因表达.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
在RNA生物学,RNA生物学.

背景情况:

已知循环RNAs (circRNAs) 与微RNAs和蛋白质相互作用.
活细胞中circRNAs和信使RNAs (mRNAs) 之间的相互作用尚未得到充分理解.

研究的目的:

开发一种用于识别完整细胞中circRNA-mRNA相互作用的新技术.
研究直接circRNA-mRNA相互作用在基因表达调节中的作用.

主要方法:

开发并使用交叉链接多A拉下RNaseR测序 (CLiPPR-seq) 技术.
利用AMT介导的近距离结合,环RNA丰富和深度测序.
进行了对补充序列的BLAST分析和拉下测试,以确认相互作用.

主要成果:

在三种细胞类型 (βTC6,C2C12,HeLa) 中确定了数百种与mRNA相互作用的circRNA.
证实了circRNAs和目标mRNAs之间的直接相互作用.
证明沉默特定的circRNAs改变了βTC6细胞中的目标mRNA表达.

结论:

CLiPPR-seq是一种用于识别circRNA-mRNA相互作用的新方法.
环RNAs和mRNAs之间的直接相互作用在调节基因表达方面发挥着作用.
这项研究为探索circRNA-mRNA混合体的功能开辟了新的途径.

更多相关视频

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

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