在ALV-J受体结合域区域的N123I突变通过增加与chNHE1的结合亲和力来增强病毒复制能力
Mengmeng Yu1, Yao Zhang1, Li Zhang1
1Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, China.
PLoS pathogens
|February 7, 2024
概括
鸟类白血病病毒亚组J (ALV-J) gp85蛋白中的特定突变 (N123I) 增强了它与的交换器1 (chNHE1) 的结合,促进了病毒的复制和分泌.
科学领域:
- * 病毒学 病毒学
- * 分子生物学 * 分子生物学
- * 禽类疾病 禽类疾病
背景情况:
- *J亚组禽类白血病病毒 (ALV-J) 是一种流行性逆转录病毒.
- *ALV-J通过将其gp85蛋白与的交换机1 (chNHE1) 受体结合,侵入宿主细胞.
- * ALV-J gp85受体结合域 (RBD) 中观察到突变,特别是在中国层菌株中,但它们的影响尚不清楚.
研究的目的:
- * 调查gp85变异在ALV-J复制和传播中的功能影响.
- *确定ALV-J gp85中的特定突变如何影响与chNHE1.1的结合.
- *阐明增强病毒受体相互作用的结构基础.
主要方法:
- *对ALV-J菌株JL08CH3-1和HPRS103复制能力进行比较分析.
- *评估gp85与chNHE1.1的结合亲和力.
- *位点定向的突变发生,引入N123I突变.
- *gp85-chNHE1相互作用的结构分析.
主要成果:
- *ALV-J菌株JL08CH3-1显示出比HPRS103更高的复制能力,与gp85变异有关.
- * JL08CH3-1 gp85与HPRS103 gp85.5相比,对chNHE1的结合显著增强.
- * 在gp85中N123I突变被确定为增加chNHE1结合的关键因素.
- * 结构分析显示,N123I增强了gp85的稳定性,相互作用接口和与chNHE1.1的键.
- *N123I突变改善了ALV-J复制,并促进了体内病毒分离.
结论:
- *ALV-J gp85中的N123I突变对于增强对chNHE1.1的受体结合至关重要.
- *这种突变显著提高了病毒复制效率,并促进了病毒的分泌.
- * 了解这些突变,可以了解ALV-J的发病和传播动态.
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