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便微生物组组成和冷保存犬的多样性在不同的持续时间和储存条件下

Patrick Barko¹, Julie Nguyen-Edquilang¹, David A Williams¹

¹Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, United States of America.

|February 7, 2024

概括

新鲜冷的狗可以在 -80°C下保存长达12个月,对微生物组成的影响最小. 储存在-80°C优于-20°C,以保持狗的便微生物群稳定性.

科学领域:

兽医微生物学兽医微生物学
微生物组研究微生物组研究
结冷保存是一种冷保存.

背景情况:

新鲜冷的便库正在为人类疗法而出现.
建立一个兽医新鲜冷便银行是当前的目标.
冷便的稳定性对于兽医便微生物群移植 (FMT) 是至关重要的.

研究的目的:

评估冷储存后狗便微生物组组成和多样性的稳定性.
为了比较20°C和80°C储存对12个月内狗便样本的影响.
为未来的兽医FMT确定狗便材料的最佳储存条件.

主要方法:

收集了20只健康狗的便,用糖醇保存,并在-20°C和-80°C下保存长达12个月.
分析了微生物社区的组成和多样性,使用长时间读取的16S-rRNA amplicon测序.
在基线和各种储存时间后比较微生物特征.

主要成果:

狗便的微生物组成和多样性在 -80°C下长达12个月保持稳定.
与-20°C相比,在-80°C储存显示出更高的稳定性.
个体狗的变异对微生物群体的影响最大.

结论:

新鲜冷的狗可以在 -80°C下可靠地存储长达12个月,用于兽医应用.
储存温度显著影响便微生物群的稳定性, -80°C是最佳温度.
需要进一步的研究来将储存诱导的微生物变化与FMT临床疗效联系起来.

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