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开发基于超氧化物脱酶的视觉和光谱测量方法,用于快速区分新鲜和冷解的水牛肉

Apeksha Jangir¹, Ashim Kumar Biswas¹, Abdullah Arsalan¹

¹Division of Livestock Products Technology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, U.P., India.

|February 7, 2024

概括

一种新的测定方法可以快速检测新鲜和冷解的水牛肉,使用超氧化物脱酶 (SOD) 作为生物标志物. 这种方法为肉类供应链验证提供了可靠的工具.

科学领域:

食品科学食品科学食品科学
生物化学生物化学
分析化学分析化学

背景情况:

确保供应链中的肉类真实性对于消费者安全和监管合规性至关重要.
区分新鲜和冷解的肉类对于质量控制和防止欺诈行为至关重要.

研究的目的:

开发一种基于生物标志物的快速测试方法,以区分新鲜的水牛肉和冷解的水牛肉.
验证测试在肉类供应链中的可靠性和适用性.

主要方法:

开发一种特定的溶剂系统,并确定最佳基质和开发者用于生物标志物选.
使用柱状染色学,凝电泳和西式斑点测试来确认生物标志物的身份.
通过日内和日间精度研究,可存储性评估和热史确定进行测试验证.

主要成果:

优化试验使用pH 8.0的Tris-HCl提取缓冲剂,尼古丁胺胺氨基二核酸 (205μM) 作为基质,和Nitroblue tetrazolium (184μM) 与phenazine甲硫酸盐 (1.9μM) 作为开发剂.
证实超氧化脱酶 (SOD) 是区分肉类状态的可靠生物标志物.
该试验显示出很好的精度,变化系数 (CV %) 在冷样本中为5.36.5%,在冷样本中为9.214.1%.

结论:

开发的基于生物标志物的测试,利用SOD,在快速检测新鲜与冷解的水牛肉方面取得了重大进展.
这种方法在整个供应链中对肉制品的监测和验证具有实践应用的巨大潜力.

关键词:

生物标志物生物标志物酶方法酶方法肉类肉类肉类超氧化物脱酶的作用是什么供应链中的供应链.

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