在胚外附录中的阿迪波涅丁和维斯法丁表达特征和在层和肉胚胎发育期间的作用
Ophélie Bernardi1, Christelle Ramé2, Maxime Reverchon3
1SYSAAF-Syndicat des Sélectionneurs Avicoles et Aquacoles Français, Centre INRAE Val de Loire, F-37380, Nouzilly, France; Centre National de la Recherche Scientifique, Institut Français du Cheval et de l'Equitation, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Université de Tours, Physiologie de la Reproduction et des Comportements, UMR85, F-37380 Nouzilly, France.
General and comparative endocrinology
|February 7, 2024
概括
阿迪波涅丁和维斯法丁的层次比肉胚胎高. 维斯法丁抗体增加了胚胎死亡率,这表明维斯法丁可以调节鸟类胚胎的发育.
科学领域:
- 禽畜科学 禽畜科学 禽畜科学
- 发展生物学 发展生物学
- 内分泌学 在内分泌学.
背景情况:
- 层和肉胚胎发育的差异被注意到.
- 阿迪波涅克丁和维斯法丁在鸟类的胚胎外膜和流体中表达.
- 这些阿迪波金在胚胎发育中的特定作用仍然在很大程度上是未知的.
研究的目的:
- 为了比较adiponectin (AdipoQ),它的受体 (AdipoR1,AdipoR2) 和visfatin表达在层和肉胚胎的胚胎外组织中.
- 通过内卵性抗体注射,调查阿迪波涅克丁和维斯法丁对鸟类胚胎发育的影响.
主要方法:
- 在胚胎发育过程中对艾迪波Q,艾迪波R1,艾迪波R2和维斯法丁在艾伦托和羊水膜和流体中的表达量的定量分析 (ED7-ED18).
- 在卵子内注射阻断性抗体,用于在层和肉胚胎中对抗阿迪波涅丁或维斯法丁.
- 在抗体治疗后评估胚胎死亡率和体重.
主要成果:
- 阿迪波涅丁,它的受体和视法丁的表达随着胚胎的发育而增加,主要是在亚兰托膜中.
- 与特定发育阶段的肉胚胎相比,层胚胎表现出更多的AdipoQ,AdipoR1,AdipoR2和visfatin的表达.
- 胚胎液中阿迪波金度与胚胎体重之间观察到正相关性. 注射维斯法丁抗体显著增加了胚胎死亡率,以剂量依赖的方式.
结论:
- 阿迪波涅丁和维斯法丁显示层和肉胚胎之间的差异性表达模式,层中的水平更高.
- 维斯法丁在调节鸟类胚胎发育方面起着至关重要的作用,抗体介导阻塞后的死亡率增加证明了这一点.
- 这些发现突显了阿迪波金在鸟类胚胎生长和存活方面的潜在意义.
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