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相关概念视频

Cell Culture01:21

Cell Culture

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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工程哺乳动物细胞生长动态用于生物制造.

Mauro Torres1, Dewi Mcconnaughie1, Samia Akhtar1

  • 1Manchester Institute of Biotechnology, Faculty of Science and Engineering, University of Manchester, Manchester, UK; Department of Chemical Engineering, Biochemical and Bioprocess Engineering Group, University of Manchester, Manchester, UK.

Metabolic engineering
|February 7, 2024
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概括

这项研究对哺乳动物细胞进行了改造,用于生物制药制造中的精确生长控制. 合成生物学工具被用来创建可调节的"气体和制动"系统来调节细胞生长动态.

关键词:
生物制药产品 生物制药产品克里斯普尔-Cas9是什么意思细胞工程是细胞工程.基因电路的基因.控制核扩散的方法

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科学领域:

  • 合成生物学 合成生物学
  • 细胞工程 细胞工程
  • 生物制药制造业 生物制药制造业

背景情况:

  • 哺乳动物细胞生长动态在生物制药生产中存在挑战.
  • 控制细胞增殖和寿命对于优化制造工艺至关重要.

研究的目的:

  • 开发一个多层次的细胞工程策略,用于调节哺乳动物细胞生长阶段的调节.
  • 创建一个双重可控制的系统,精确编排细胞生长动态.

主要方法:

  • 通过CRISPR/Cas9基因编辑,淘汰亲亡蛋白 (Bax和Bak) 以延长寿命.
  • 酸诱导系统调节cMYC以加速生长.
  • 环素可诱导的基因电路调节BLIMP1以阻止生长.

主要成果:

  • 工程细胞显示减弱了亡,提高了活力,延长了培养寿命.
  • 通过增长加速系统实现了细胞密度的快速增加和细胞周期的控制.
  • 使用静止相诱导系统证明了细胞生长停止和细胞循环停止.
  • 开发了一种用于动态和精确控制哺乳动物细胞生长阶段的双重系统.

结论:

  • 开发的战略为操纵哺乳动物细胞生长提供了一个复杂的框架.
  • 这种方法通过精确控制细胞行为来增强生物制药制造.
  • 合成生物学工具和组合工程为生物医学应用提供了一个新的范式.