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Updated: Aug 1, 2026

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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技术报告:临床上有用的蛋白质选择协议:用于确定临床上有用的蛋白质以进行验证的方法

Ella E K Swaney1, Stephen Hearps2, Paul Monagle3

  • 1Haematology Group, Murdoch Children's Research Institute, 50 Flemington Road, Parkville, Melbourne 3052, Australia; Department of Paediatrics, University of Melbourne, Melbourne 3050, Australia.

Journal of proteomics
|February 7, 2024
PubMed
概括

临床有用的蛋白质选择 (CUSP) 协议通过优先考虑临床适用性来增强蛋白质标记物的发展. 这确保了新的疾病标志物可以很容易地转化为实际的医疗保健环境.

关键词:
生物标志物 生物标志物数据处理和分析数据.有针对性的蛋白质组学.

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相关概念视频

Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.